Dilution of the solution samples: The actual concentration of the protein in the samples: The amino acids are joined together by dehydration synthesis to forms chains, which are hundreds of amino acids long which is called proteins.
Saponification of triglyceride The saponification number sap measures the bonded and unbonded acids present in an oil or fat.
It defines the exact amount of potassium hydrate in mg necessary to emulsify 1g of fat or oil. The smaller the molar mass of the fat, the higher the saponification value.
Different group used different oil which is palm oil and corn oil. Then at the end of the experiment we obtained the final results for each sample.
Based on the results obtained, sunflower oil has the highest saponification number compared to corn oil and palm oil.
So it shown that, sunflower oil has shorter fatty acids. Theory said that triglycerides containing long fatty acids will have a lower saponification number than triglycerides with shorter fatty acids.
Since 1 gram of lipid containing long chains will have less chains in total than 1 gram of lipid containing short chains. Actually, we should get the highher saponification number for palm oil followed by corn oil and the lowest is sunflower oil.
Low fatty acid fats like coconut oil or palm kernel fat have high saponification numbers ofwhereas most vegetable oils have a saponification number of approximately It means that, palm oil have the shorter fatty acid chain than corn oil and sunflower oil. For the palm oil and corn oil, the saponification number should higher than sunflower oil because they have shorter fatty acid chain than sunflower oil.
A few error occured during the experiment that effect the results obtained that are, there is no standard colour of solution when turns colourless.
So, this affect the titration process. It is an exothermic chemical reaction which happens when fatty acids react with base. This process involves boiling the fats together with the base. For this experiment, we boiled sunflower oil with 6M NaOH. The hydrolysis of the oil occurs, thus producing glycerol and crude soap.
Then, sodium chloride is added into it once the saponification reaction completed. Addition of sodium chloride causes the soap to precipitate.
The crude soap acquired from the process consists of sodium chloride, sodium hydroxide and glycerol.
The crude soap curds is then boiled in water and re-precipitated with salt in order to remove the impurities. After that, we add some color into it and the result is as the picture below. In saponification, boiling triglycerides oils react with concentrated alkali potassium hydroxide to produce glycerol and salts of fatty acids.
Triglycerides exist in the organic phase while the hydroxides exist in the aqueous phase. By increasing agitation, the reactants will happen to be in the same phase. Therefore, the oils can be hydrolyzed to form glycerol and fatty acids. The acids then react with potassium hydroxide to form potassium salts.
Thus, glycerol and fatty acid salts that have appreciable solubility in both phases can be formed. The reagents will increase in concentration in the phase which is most favored by the other as the reaction progresses.
Why do triglycerides with longer fatty acids have a lower saponification number than those with shorter fatty acids?May 21, · This experiment is carried on in order to evaluate one of several triglycerides to determine the saponification number.
This experiment also been carried out to make soap by the same process called the saponification, using the modern ingredient. The experiment to determine the saponification had been carried out in the fume hood.
welcome to bio chem technology laboratory, inc. p.o. box yonkers, ny In this experiment you will learn several ways in which proteins are coagulated; also includes a quiz.
(Submitted by Rita Snyder) Root Beer Production - This experiment will illustrate to the student that fermentation processes, as in yeast fermentation can be used to . welcome to bio chem technology laboratory, inc.
p.o. box yonkers, ny ACCU Bio-Chem Laboratories is an independent analytical lab. Our expertise includes but is not limited to Cosmetics and OTC products. A few of our many Specialty services include Analytical analysis, Microbial analysis, Stability Studies, procedure development and purified water set-up including validation for manufacturing plants.
Clinical chemistry (also known as chemical pathology, clinical biochemistry or medical biochemistry) is the area of chemistry that is generally concerned with analysis of bodily fluids for diagnostic and therapeutic purposes.
It is an applied form of biochemistry (not to be confused with medicinal chemistry, which involves basic research for drug development).